Role of polyol pathway in nonenzymatic glycation

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Role of polyol pathway in nonenzymatic glycation.

In order to confirm the link between nonenzymatic glycation and the polyol pathway, we observed the effect of treatment with epalrestat (Ep), an aldose reductase inhibitor, on the concentration of advanced glycation end-products (AGEs) in erythrocytes from diabetic patients. We also examined the effect of the drug on erythrocyte fructose 3-phosphate (F3P), a novel metabolite that has been repor...

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The Role of the Polyol Pathway

an increase in the red cell's normal substrate, glucose, can produce changes in red cell metabolism.

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The role of nonenzymatic glycation and carbonyls in collagen cross-linking for the treatment of keratoconus.

PURPOSE Corneal cross-linking (CXL) is a treatment for keratoconus that eliminates the need for keratoplasty in most patients. However, its molecular mechanisms remain under study. Advanced glycation end products (AGEs) have been suggested by many studies as the causative strengthening agent during CXL, though no studies to date have directly tested this hypothesis. METHODS Corneas of young r...

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Study of Nonenzymatic Glycation of Transferrin and its Effect on Iron -Binding Antioxidant Capacity

Objective(s) Nonenzymatic glycosylation (glycation) occurs in many macromolecules in aging and diabetes due to exposure of biomolecules to high level of glucose. Glycation can changes function, activities and structure of many biomolecules. Considering this important role of transferrin (Trf) in iron transport and antioxidant activity in plasma this study was carried out to investigate the eff...

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Nonenzymatic glycation of immunoglobulins does not impair antigen-antibody binding.

We glycated immunoglobulins from commercial kits designed to measure human ferritin, thyrotropin, and transferrin, and compared the calibration curves for assays utilizing glycated antibodies with those of assays utilizing non-glycated antibodies. Glycation was verified by borate affinity chromatography and assay with thiobarbituric acid reagent. We found no evidence that antigen-antibody bindi...

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ژورنال

عنوان ژورنال: Nephrology Dialysis Transplantation

سال: 1996

ISSN: 0931-0509,1460-2385

DOI: 10.1093/ndt/11.supp5.95